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[目的 ]对 0 .9%苏·阿可湿性粉剂进行急性毒性试验 ,为农药登记提供毒性资料。 [方法 ]按照GB15 6 70 1995《农药登记毒理学试验方法》进行。 [结果 ]急性经口毒性试验 ,剂量分别以2 0 0 0、2 715、36 85、5 0 0 0mg/kg给予。将样品空腹灌胃 0 5h后 ,雌雄大鼠均有对外界刺激反应迟钝、抽搐、麻痹、四肢瘫痪、眼球微量出血、腹泻等中毒表现 ,2 4h后多数实验动物逐渐恢复正常。 2 715、36 85、5 0 0 0mg/kg剂量组 ,分别有 2、4、5只雄性大鼠死亡 ,而雌性大鼠各剂量组无一只动物死亡。经计算 ,雄性大鼠急性经口LD50 为 2 974 4mg/kg ,95 %可信区间为 2 5 11 31～ 35 2 2 9mg/kg ;雌性大鼠急性经口LD50 大于 5 0 0 0mg/kg ;雌雄大鼠急性经皮毒性试验 ,涂敷 2 0 0 0mg/kg无任何不良反应 ,LD50 均大于 2 0 0 0mg/kg ;样品 5 0 %糊状受试物一次接触动物皮肤 ,对家兔皮肤刺激反应积分均值为 0 ,属无刺激性 ;样品 10 0倍稀释液 ,不冲洗的条件下 ,对家兔眼睛平均刺激指数为 0 ,属无刺激性 ;样品 5 0 %糊状受试的在皮肤多次致敏接触实验动物 ,并经激发后未见动物有红斑、水肿过敏反应 ,致敏率为 0 ,表明该受试物属弱致敏物。 [结论 ] 0 9苏·阿可湿性粉剂属低毒农药 ;对实验动物皮肤及粘膜无刺激性 ;不引起实?
[Objective] The acute toxicity test of 0.9% SOHU WP was conducted to provide toxicity information for pesticide registration. [Method] According to GB15 6 70 1995 “Toxicological test of pesticide registration”. [Results] Acute oral toxicity test, the dose was 200, 2715, 36 85, 500 mg / kg. The samples were fasting gavage 0 5h, both male and female rats to the outside world to stimulate the unresponsive, convulsions, paralysis, limb paralysis, eyeballs bleeding, diarrhea and other poisoning performance, most of the experimental animals returned to normal after 24 h. 2 715,36 85,5 0 0 0 mg / kg dose group, respectively, 2, 4, 5 male rats died, while the female rats in each dose group, no animal died. The calculated acute oral LD50 of male rats was 2 974 4 mg / kg with 95% confidence interval of 2 5 11 31 to 35 2 2 9 mg / kg. The acute oral LD50 of female rats was more than 500 mg / kg. Male and female rat acute dermal toxicity test, application of 2000 mg / kg without any adverse reactions, LD50 were greater than 2000 mg / kg; sample 50% paste test a contact with animal skin, rabbit skin The mean value of irritation response score is 0, which is non-irritating. The average eye irritation index of rabbits is 0, which is non-irritating when the sample is diluted with 10 times dilution. Skin sensitization repeated exposure to experimental animals, and after the stimulation of animals have no erythema, edema allergic reaction, the sensitization rate of 0, indicating that the test is weak allergens. [Conclusion] 0 9 SU · WP is a low-toxic pesticide; it is non-irritating to the skin and mucous membrane of experimental animals;