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目的:比较重症肌无力患者胸腺与正常胸腺中CD53与胸腺细胞亚群的差异,探讨重症肌无力患者胸腺异常增生的机制。方法:采用流式细胞术分析重症肌无力患者胸腺与正常胸腺(对照组)中胸腺细胞CD4、CD8、CD45RA、CD45RO的表达,采用实时荧光定量PCR技术检测重症肌无力患者和对照组胸腺组织CD53mRNA的表达水平。结果:重症肌无力患者胸腺双阳性细胞(CD4+CD8+)、单阳性细胞(CD4+CD8-和CD4-CD8+)均高于对照组差异有统计学意义(P<0.01)。CD45RA分子表达明显低于对照组(P<0.01),CD45RO分子表达明显高于对照组(P<0.01)。荧光定量PCR发现CD53mRNA的相对表达量在重症肌无力患者胸腺组织中为(4.45±0.5),对照组为(5.58±3.140),与对照组相比较,重症肌无力患者胸腺组织中CD53mRNA的表达上调2.66倍。结论:CD53与重症肌无力患者胸腺细胞亚群异常分布有关,可能与重症肌无力胸腺异常及其发病机制有关。
Objective: To compare the differences of CD53 and thymocyte subsets between thymus and normal thymus in patients with myasthenia gravis, and to explore the mechanism of thymus dysplasia in patients with myasthenia gravis. Methods: The expressions of CD4, CD8, CD45RA and CD45RO were detected by flow cytometry in thymic thymus and normal thymus (control group). The expression of CD53 mRNA in thymic tissue of patients with myasthenia gravis and control group was detected by real-time fluorescence quantitative PCR The level of expression. Results: The levels of CD4 + CD8 +, CD4 + CD8- and CD4-CD8 + in thymus patients with myasthenia gravis were significantly higher than those in controls (P <0.01). The expression of CD45RA was significantly lower than that of the control (P <0.01), and the expression of CD45RO was significantly higher than that of the control (P <0.01). Fluorescent quantitative PCR revealed that the relative expression level of CD53 mRNA was (4.45 ± 0.5) in thymic tissue of myasthenia gravis and (5.58 ± 3.140) in control group, and the expression of CD53 mRNA in thymus tissue was increased in patients with myasthenia gravis 2.66 times. Conclusion: CD53 is associated with the abnormal distribution of thymocyte subsets in patients with myasthenia gravis, which may be related to the abnormality of myasthenia gravis and its pathogenesis.