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用原位杂交和荧光免疫定位方法研究了组织型（ｔ）和尿激酶型（ｕ）纤溶酶原激活因子ｔＰＡ、ｕＰＡ和相应的抑制因子ＰＡＩ－１、ＰＡＩ－２在人和恒河猴胎盘中的定位和分布。结果表明：（１）激活因子ｔＰＡ、ｕＰＡ（Ｆｉｇ．１＆４）和抑制因子ＰＡＩ－１（Ｆｉｇ．２）、ＰＡＩ－２（Ｆｉｇ．３）一般都在不同程度上定位于两者胎盘的相同部位；（２）它们主要分布在绒毛干和蜕膜的血管壁、 ＲＯｈｒ’ｓ和Ｎｉｔａｂｕｃｈ’ｓ纹间的基盘外绒毛滋养层细胞、滋养壳、蜕膜细胞和腺体细胞。并且发现；ｔＰＡ和它的抑制因子ＰＡＩ－１更明显地定位于邻近母体组织离层界面的区域，而ｕＰＡ和抑制因子ＰＡＩ－１更集中在绒毛滋养层和外绒毛滋养细胞中；（３）激活因子和抑制因子的ｍＲＮＡ和蛋白的定位和分布基本上一致，但是、在绒毛核体滋养层细胞上未发现其ｍＲＮＡ表达，却有很强的免疫荧光的分布；（４）激活因子和抑制因子合成和分布部位与其作用底物，即纤蛋白类分子的产生部位一致；（５）未发现上述分子在人和恒河猴胎盘分布上的不同。上述实验结果说明，在妊娠的各个阶段 ＰＡ和它的抑制因子协同表达，局限在作用范围很小的特定产生底物的区域。它们的相互作用可能是维持正常妊娠所必需。在妊?
Tissue type (t) and urokinase (u) plasminogen activator tPA, uPA and corresponding inhibitors PAI-1 and PAI-2 were studied in human and rhesus monkeys by using in situ hybridization and fluorescence immunolocalization Positioning and distribution in the placenta. The results showed that: (1) The activators tPA, uPA (Fig.1 & 4) and PAI-1 (Fig.2) and PAI-2 ; (2) They are mainly distributed in the vascular wall of the hair follicle and decidua, basal disc outer trophoblast cells, nourishment shells, decidual cells and glandular cells between ROhr’s and Nitabuch’s moles. And found that tPA and its inhibitor PAI-1 were more localized in the region close to the delaminated interface of maternal tissue, while uPA and PAI-1 were more concentrated in trophoblast and villous trophoblast; (3) The localization and distribution of mRNA and protein of activation and inhibitory factors were basically the same, but no mRNA expression was found on trophoblast cells of villi nucleus, but there was a strong distribution of immunofluorescence. (4) Activation factor and inhibition Factor synthesis and distribution sites and its role in the substrate, that fibrin molecules generated parts consistent; (5) did not find the above-mentioned molecules in human and rhesus placenta distribution differences. The above experimental results show that PA and its inhibitory factor co-expression at various stages of pregnancy is limited to a specific substrate-generating region of small effect. Their interaction may be necessary to maintain normal pregnancy. Pregnancy?