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本实验采用离体血管灌流方法，观察和比较血管钠肽（VNP）、C型钠尿肽（CNP）和心房钠尿肽（ANP）对大鼠肺动脉、腹主动脉和腹腔静脉的舒张作用。结果表明，VNP、CNP和ANP对离体大鼠的保留内皮与去内皮的肺动脉、腹主动脉和腹腔静脉均有浓度依赖性舒张作用。对内皮完整的肺动脉、腹主动脉和腹腔静脉的最大舒张反应（Rmax），VNP分别为（76±17）％，（51±14）％和（62±14）％；CNP分别为（31±8）％，（22±7）％和（41±8）％；ANP分别为（38±10）％，（41±10）％和（11±4）％。内皮完整的肺动脉、腹主动脉和腹腔静脉最大舒张的半数有效浓度（EC50），VNP分别为16±11nmol/L，35±18nmol/L和12±8nmol/L；CNP分别为148±112nmol/L，299±84nmol/L和14±12nmol/L，；ANP分别为66±47nmol/L，16±15nmol/L和909±445nmol／L。VNP的舒张作用显著强于CNP和ANP，有显著差异（P＜0．05～0．01）。但在保留内皮组与去内皮组间无显著差异（P＞0．05）。松弛肺动脉的作用依次为VNP＞ANP≥CNP；松弛腹主动脉的作用依次为VNP＞ANP＞CNP；松弛腹腔静脉的作用依次为VNP＞CNP＞ANP。以上结果提示，VNP的舒血管作用显著强于CNP和ANP，它是一个新合成的、强效的、非内皮依赖性的血管松弛多肽，较CNP和ANP更具有潜?
In this study, the vasodilatory effects of vasopressin (VNP), C-type natriuretic peptide (CNP) and atrial natriuretic peptide (ANP) on pulmonary arteries, abdominal aorta and celiac vein were observed and compared in vitro. The results showed that VNP, CNP and ANP had a concentration-dependent relaxation effect on isolated rat endothelium and endothelium -associated pulmonary artery, abdominal aorta and celiac vein. The maximal vasodilation response (Rmax) and VNP were (76 ± 17)%, (51 ± 14)% and (62 ± 14)%, respectively, for intact pulmonary artery, abdominal aorta and celiac vein; 8%, 22 ± 7%, and 41 ± 8%, respectively; ANP was (38 ± 10)%, (41 ± 10)% and (11 ± 4)%, respectively. The median effective concentration (EC50) of maximal vasodilatation of pulmonary arteries, abdominal aorta and celiac vein were 16 ± 11 nmol / L, 35 ± 18 nmol / L and 12 ± 8 nmol / L, respectively. The CNP were 148 ± 112 nmol / L , 299 ± 84 nmol / L and 14 ± 12 nmol / L, respectively; ANP was 66 ± 47 nmol / L, 16 ± 15 nmol / L and 909 ± 445 nmol / L respectively. The relaxation of VNP was significantly stronger than that of CNP and ANP (P <0.05 ~ 0.01). However, there was no significant difference between the endothelium group and the endodermis group (P> 0.05). The effects of relaxation of the pulmonary artery were VNP> ANP≥CNP, relaxation of the abdominal aorta followed by VNP> ANP> CNP, relaxation of the celiac vein followed by VNP> CNP> ANP. The above results suggest that vasodilator effect of VNP is significantly stronger than that of CNP and ANP. It is a newly synthesized, potent and non-endothelium-dependent vasodilator polypeptide, which is more latent than CNP and ANP.