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目的:了解广东省副溶血性弧菌水产品分离株与食物中毒分离株携带毒力基因和耐药情况及差异。方法:对132株副溶血性弧菌用纸片扩散法进行耐药检测,对487株副溶血性弧菌以PCR方法进行毒力基因测定。结果:副溶血性弧菌菌株对14种抗生素都有耐药。同时对5种以上抗生素耐药的有60株,占45.5%。多重耐药株的构成比以海虾分离株和食物中毒株为主,占50%。耐药谱分析结果显示,副溶血性弧菌分离株对青霉素类、庆大霉素、连霉素等,普遍有较高的耐药率。487株副溶血性弧菌的tdh和trh基因检测,食品分离株毒力基因的携带率明显低于食物中毒分离株。而食物中毒分离株中以携带tdh基因为多,占93.75%。结论:为防止超级耐药株的产生,应尽快立法加强对兽药使用的监控。复合PCR基因检测能缩短检测周期,有应用前景。
Objective: To understand the virulence genes and drug resistance of isolates of Vibrio parahaemolyticus isolates and food poisoning isolates from Guangdong Province. Methods: 132 strains of Vibrio parahaemolyticus were detected by disk diffusion method. Virulence genes of 487 Vibrio parahaemolyticus were detected by PCR. Results: Vibrio parahaemolyticus strains were resistant to all 14 antibiotics. There were 60 strains resistant to more than 5 antibiotics, accounting for 45.5%. The composition of multidrug-resistant strains than shrimp isolates and food poisoning strains, accounting for 50%. Resistant spectrum analysis showed that Vibrio parahaemolyticus isolates against penicillins, gentamicin, clindamycin, etc., generally have a higher resistance rate. 487 isolates of Vibrio parahaemolyticus tdh and trh gene detection, food isolates virulence gene carrier rate was significantly lower than food poisoning isolates. The food poisoning isolates to carry more tdh gene, accounting for 93.75%. Conclusion: To prevent the emergence of super-resistant strains, legislation should be strengthened to monitor the use of veterinary drugs as soon as possible. Complex PCR gene test can shorten the detection cycle, there are prospects.