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160.泻药中番泻甙A和B的高压液相色谱分析番泻甙A和B互为同分异构,常混合存在。采用最常用的分光光度法很难将它们区别和进行单一成分的含量测定。本文报道番泻粉末,番泻果实片和番泻甙片中主要蒽醌甙即番泻甙A和B的HPLC定量分析方法。间接HPLC定量分析:标准溶液的配制:精确称取番泻甙A和B标准品各2毫克,分别加至1毫升正丙醇-乙酸乙酯-水
160. High Pressure Liquid Chromatographic Analysis of Senna Pi A and B in Laxatives Sennae A and B are isomeric and often mixed. Using the most commonly used spectrophotometric methods it is difficult to distinguish them and perform single-component determinations. This paper reports the HPLC quantitative analysis methods of senna, powdered granum and granules of Panaxa Aconitum and Panaxa Radix, which are the main glycosides in Panaxa Aconitum. Indirect HPLC Quantitative Analysis: Preparation of Standard Solution: Accurately weigh 2 mg of each of the senna valeria A and B standards and add them to 1 ml of n-propanol-ethyl acetate-water.