论文部分内容阅读
目的探讨人参皂苷Rb1在大鼠脑创伤后的脑水含量及血清S100β的影响。方法参照Feeney MD等的方法建立自由落体颅脑外伤模型。将24只SD雄性大鼠随机分为假手术组、模型组、人参皂苷Rb1低剂量组、人参皂苷Rb1高剂量组,每组6只。假手术组只做开骨窗处理;人参皂苷Rb1低、高剂量组于脑创伤后即刻腹腔注射相应剂量的人参皂苷Rb1注射液;模型组在创伤后即刻腹腔注射等量生理盐水。采用干湿比重法测脑水含量,酶联免疫吸附ELISA法检测血清中S100β的质量浓度变化。结果与假手术组比较,其余3组脑含水量和血清S100β增加,有显著差异;与模型组比较,人参皂苷Rb1低、高剂量组脑含水量和血清S100β降低,有显著差异;人参皂苷Rb1高剂量组血清S100β较低剂量组显著降低。结论腹腔注射人参皂苷Rb1可减少脑创伤24 h后大鼠的脑含水量、降低血清S100β含量,提示注射人参皂苷Rb1对脑创伤后大鼠具有脑保护作用,且与剂量有关。
Objective To investigate the effect of ginsenoside Rb1 on brain water content and serum S100β after traumatic brain injury in rats. Method According to Feeney MD and other methods to establish free fall cranial trauma model. 24 SD male rats were randomly divided into sham operation group, model group, low dose of ginsenoside Rb1 group and high dose of ginsenoside Rb1 group, 6 rats in each group. The rabbits in the sham operation group were treated with open-skull window only. The rats in the low and high dose ginsenoside Rb1 groups were injected intraperitoneally with the corresponding dose of ginsenoside Rb1 injection immediately after traumatic brain injury. The rats in the model group were given intraperitoneal injection of normal saline immediately after trauma. The content of brain water was measured by dry weight and wet weight, and the concentration of S100β in serum was detected by enzyme-linked immunosorbent assay (ELISA). Results Compared with the sham-operation group, the brain water content in the remaining three groups and the serum S100β increased significantly. Compared with the model group, the brain water content of the low and high dose ginsenoside Rb1 and the serum S100β decreased significantly. The ginsenoside Rb1 High-dose serum S100β lower dose group was significantly lower. Conclusion Intraperitoneal injection of ginsenoside Rb1 can reduce the brain water content of rats after 24 h of traumatic brain injury and decrease the content of S100β in serum, which indicates that ginsenoside Rb1 can protect the brain from traumatic brain injury and dose-related.