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目的:探讨趋化因子Fractalkine对脂多糖(LPS)诱导的小鼠小胶质细胞(N9)激活时所分泌的TNF-α、IL-1β和一氧化氮(NO)表达的影响。方法:用Fractalkine处理经LPS激活的小鼠小胶质细胞24 h,以ELISA法检测细胞培养上清中TNF-α和IL-1β的浓度,以NO试剂盒检测培养上清中NO的浓度。结果:LPS能够激活小胶质细胞,使IL-1β、TNF-α和NO的表达量与对照组相比明显升高;Fractalkine能够降低LPS激活的小胶质细胞IL-1β、TNF-α和NO的表达。结论:Fractalkine可能通过抑制炎症相关因子的产生而在中枢神经系统中发挥神经保护作用。
AIM: To investigate the effect of fractalkine on the expression of TNF-α, IL-1β and nitric oxide (NO) secreted by lipopolysaccharide (LPS) -induced mouse microglial cells (N9) activation. METHODS: LPS-activated mouse microglia cells were treated with Fractalkine for 24 h. The concentrations of TNF-α and IL-1β in the cell culture supernatants were detected by ELISA. NO concentration in the culture supernatant was detected by NO kit. RESULTS: LPS activated microglial cells and significantly increased the expression of IL-1β, TNF-α and NO compared with the control group. Fractalkine reduced the levels of IL-1β, TNF-α and NO expression. Conclusion: Fractalkine may play a neuroprotective role in the central nervous system by inhibiting the production of inflammation-related factors.