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在pH4.8的NaAc HAc缓冲液中,钍试剂Ⅰ与蛋白质能够相互作用形成超分子复合物,使钍试剂Ⅰ在-0.58V(SCE)处的极谱还原峰峰电流下降,在最佳条件下,峰电流的下降值同牛血清白蛋白(BSA)的质量浓度在1.5～25mg/L范围内呈线性关系,其线性回归方程为Δip=-265.4+181.2ρBSA,相关系数r=0 998(n=7),检出限为1mg/L。可应用于血清样品的测定。
In pH 4.8 NaAc HAc buffer solution, thoron I interacted with the protein to form a supramolecular complex, which decreased the peak current of the polarographic peak of thoron I at -0.58V (SCE). Under the optimal conditions , The decrease of peak current was linear with the mass concentration of bovine serum albumin (BSA) in the range of 1.5-25 mg / L. The linear regression equation was Δip = -265.4 + 181.2 ρBSA with a correlation coefficient of r = 0 998 n = 7), the detection limit was 1mg / L. Can be applied to the determination of serum samples.