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目的 了解CD4 4内含子 9、CD4 4v6mRNA在恶性脑肿瘤中的表达水平及临床意义。方法 应用RT PCR技术检测 2 8例恶性脑膜瘤 ,2 5例脑神经胶质瘤及 2 0例脑瘤旁组织中CD4 4内含子 9、CD4 4v6mRNA的表达并对mRNA含量进行半定量分析。 结果 CD4 4v6mRNA在恶性脑膜瘤和脑神经胶质瘤的阳性表达率分别为 92 9% ( 2 6/ 2 8)和 88 0 % ( 2 2 / 2 5 ) ,明显高于瘤旁组织的 5 %( 1/ 2 0 ) ,差异有非常显著性意义 (P <0 0 1) ,前二者相对含量 (mass)值明显高于瘤旁组织。恶性脑膜瘤和脑神经胶质瘤CD4 4内含子 9mRNA表达水平明显高于瘤旁组织 (P <0 0 1)。 结论 CD4 4内含子 9、CD4 4v6mRNA对辅助诊断恶性脑肿瘤有一定的意义。
Objective To investigate the expression of CD4 4 intron 9 and CD4 4v6 mRNA in malignant brain tumors and its clinical significance. Methods RT-PCR was used to detect the expression of CD4 4 intron 9 and CD4 4 v6 mRNA in 28 malignant meningiomas, 25 gliomas and 20 para-cancerous tissues. Semi-quantitative analysis of mRNA levels was performed. Results The positive rates of CD4 4v6 mRNA in malignant meningiomas and gliomas were 92 9% (2 6/2 8) and 88 0% (2 2/2 5) respectively, which were significantly higher than those in 5% (1/2 0), the difference was significant (P <0.01), the former two relative mass (mass) value was significantly higher than the tumor-adjacent tissue. The expression of CD4 intron 9 mRNA in malignant meningiomas and gliomas was significantly higher than that in tumor-adjacent tissues (P <0.01). Conclusion The CD4 4 intron 9 and CD4 4 v6 mRNA have certain significance in the diagnosis of malignant brain tumors.