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Thepathogenesisof prem ature ovarian failure (POF) rem ains unknow n. Accu- m ulated evidences indicate that abnorm ality in the im m une system m ay be one of the m ajor reasonsand theim balanceof Thelp typeI(Th1) and Thelp typeII(Th2) m ay play an importantrolein theprocess. Study of thedistribution and functionalstatusof Th1/Th2 m ay behelpfulforevaluating thepathogenesisof POF.13 patientsw ith id- iopathic POFand 7 w om en of reproductiveagew ith norm alcyclew ereenrolled in this study.The percentage of Th1 and Th2 cellsfrom peripheralblood of thepatientsand the controls w as studied using FITC labelled CD4 m Ab to separate CD4+ cells by FACS, in com bination w ith in situ hybridization using Dig-labelled IL2, IL6 cDNA probes.Results:The patients w ith POF had significantly higher percentage of Th1 cellsascompared w ith the controls(49.76±9.22,20.06±7.10respectively, P< 0.001).The ratio of Th1/Th2 in thepatientsw ith POFw assignificantly higherthan thatof thecontrols(1.15±0.17, 0.63±0.09, P< 0.001). Conclusions: Thefindings of thisstudy suggestthatpatientsw ith POFhaveincreased Th1 cellactivation, w hich m ay be related to the pathogenesisof POF. Corresponding author: WANG Yi-li E-m ail: w yl@ irix. xam u. edu. cn
The pathogenesis of prem ature ovarian failure (POF) rem ains unknow n. Accu-m ulated evidences indicate that abnorm ality in the im m une system m ay be one of the mjorjor reasons and theim balance of Thelp type I (Th1) and Thelp type II (th2) m ay play an importantrole in the process. Study of the distribution and functional status of Th1 / Th2 m ay behelpfulforevaluating the pathogenesis of POF.13 patientsw ith id-iopathic POF and 7 w om en of reproductive age w ith norm alcyclew ereen rolled in this study. percentage of Th1 and Th2 cells from peripheralblood of thepatients and the controls w as studied using FITC labeled CD4 m Ab to separate CD4 + cells by FACS, in com bination w ith in situ hybridization using Dig-labeled IL2, IL6 cDNA probes. Results: The patients w ith POF had significantly higher percentage of Th1 cells were comparable to controls (49.76 ± 9.22 °, 20.06 ± 7.10 °respectively, P <0.001). The ratio of Th1 / Th2 in thepatientsw ith POFw assignificantly higherthan that of thecon trols (1.15 ± 0.17, 0.63 ± 0.09, P <0.001). Conclusions: The findings of this research suggest that patients with invasive POF established Th1 cellactivation, w hich m ay be related to the pathogenesis of POF. Corresponding author: WANG Yi-li Em ail: w yl @ irix. xam u. edu. cn