论文部分内容阅读
目的:研究TRAIL对卵巢癌COC1/DDP细胞生长的影响,以及化疗药物DDP等对TRAIL受体(DR4、DR5)表达的影响,揭示TRAIL与COC1/DDP细胞顺铂耐药性的关系。方法:用MTT法检测不同浓度TRAIL蛋白和TRAIL与DDP联合用药对COC1/DDP细胞生长的影响,用RT-PCR方法检测DDP对TRAIL受体(DR4、DR5)表达的影响。结果:①TRAIL蛋白对COC1/DDP细胞生长有抑制作用,且随着TRAIL蛋白浓度升高,细胞抑制率逐渐上升。②DDP(2.5μg/mL)对COC1/DDP细胞生长抑制作用较弱(抑制率为3.31%),DDP在加入TRAIL蛋白后对细胞生长抑制率显著升高(P<0.05)。③DDP使COC1/DDP细胞的DR5表达水平显著增强为正常对照组的3.54倍(P<0.001)。结论:TRAIL蛋白对COC1/DDP细胞生长有抑制作用,DDP与TRAIL联合使用COC1/DDP细胞生长抑制更明显,TRAIL可逆转COC1/DDP细胞对DDP的耐药性,耐药性的逆转可能与DDP导致TRAIL受体DR5水平增高促进了肿瘤细胞的凋亡有关。
OBJECTIVE: To study the effect of TRAIL on the growth of COC1 / DDP cells and the effect of chemotherapy drug DDP on the expression of TRAIL receptor (DR4, DR5) and reveal the relationship between TRAIL and cisplatin resistance in COC1 / DDP cells. Methods: MTT assay was used to detect the effect of different concentrations of TRAIL protein and combination of TRAIL and DDP on the growth of COC1 / DDP cells. The effect of DDP on the expression of TRAIL receptor (DR4 and DR5) was detected by RT-PCR. RESULTS: (1) TRAIL protein had an inhibitory effect on the growth of COC1 / DDP cells, and with the increase of TRAIL protein concentration, the cell inhibition rate increased gradually. DDP (2.5μg / mL) had a weak inhibitory effect on the growth of COC1 / DDP cells (the inhibitory rate was 3.31%). The inhibitory rate of DDP after adding TRAIL protein was significantly increased (P <0.05). DDP significantly increased DR5 expression in COC1 / DDP cells by 3.54-fold (P <0.001) compared with that in the normal control group. CONCLUSION: The TRAIL protein can inhibit the growth of COC1 / DDP cells. The combination of DDP and TRAIL inhibits the growth of COC1 / DDP cells more significantly. TRAIL can reverse the resistance of COC1 / DDP cells to DDP. The reversal of drug resistance may be related to DDP Resulting in increased levels of TRAIL receptor DR5 promote the apoptosis of tumor cells.