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目的 研究反义c myc寡聚脱氧核苷酸 (ODN)对大鼠自体移植静脉内膜增生的影响。方法 移植静脉外周涂以加有反义c myc寡聚脱氧核苷酸的F12 7凝胶 ,于术后 1、2周测量静脉内膜平均厚度及观察血管内膜内c myc蛋白表达情况。结果 手术后 1、2周 ,移植静脉内膜的平均厚度、内膜中c myc蛋白表达在反义c myc寡聚脱氧核苷酸组均减少 ,并存在量效关系 (P <0 .0 1)。而只用F12 7胶组及正义寡聚脱氧核苷酸组均无抑制作用。结论 反义c myc寡聚脱氧核苷酸对移植静脉内膜增生有抑制作用 ,并存在量效关系。此作用可能是通过抑制血管内膜内细胞c myc蛋白的表达来实现的。
Objective To investigate the effect of antisense c myc oligodeoxynucleotide (ODN) on intimal hyperplasia of autologous vein graft in rats. Methods F127 gel with antisense c myc oligodeoxynucleotides was applied to peripheral vein of vein grafts. The average thickness of vein intima was measured at 1 and 2 weeks after operation, and the expression of c-myc in the intima was observed. Results The average intima-vein thickness and the expression of c-myc protein in the intima were decreased in the antisense c myc oligodeoxynucleotides group at 1 and 2 weeks after operation, and there was a dose-response relationship (P <0.01) ). However, only the F127 gel group and the sense oligodeoxynucleotide group had no inhibitory effect. Conclusion Antisense c myc oligodeoxynucleotides inhibit the intimal hyperplasia of vein grafts, and there is a dose-response relationship. This effect may be achieved by inhibiting the expression of c-myc protein in vascular intima.