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目的 观察不同成熟期大鼠反复惊厥后海马结构的变化 ,并研究发育鼠对惊厥性损伤的耐受性。方法 对生后 10、60d(P10、P60 )实验组大鼠用戊四氮反复点燃 5d ,设P10、P60生理盐水对照组 ;观察两实验组惊厥潜伏期、潜伏发作期、惊厥持续时间 ;海马神经元形态学改变 :海马CA1 、CA3、DG及门区进行细胞计数 ;组织化学染色观察苔藓纤维发芽 ,分别进行实验组间、实验组与对应对照组间比较。结果 1.P10惊厥发作潜伏期 (1.0 7± 0 .5 5 )min、潜伏发作期 (14 .0 0± 2 .70 )min较P60 [(8.2 7± 1.48)、(4 .16± 5 .2 2 )min]短 ,惊厥持续时间 (4 6.3 3± 7.65 )min较成年鼠 (17.0 7± 5 .66)min明显延长 ;2 .P10实验组与对照组比较海马齿状回CA1 、CA3区无明显神经元丢失 ,齿状回DG区颗粒细胞数实验组 (2 3 .2 5± 3 .0 6)较对照组 (16.2 5± 1.5 8)增多。而P60实验组CA1 、CA3区神经元计数 (8.2 2± 1.88、5 .62±l.68)较对照组 (6.3 1± 1.5 0、3 .62± 1.40 )明显减少 ,DG区无明显改变 :3 .Timm染色CA3区锥体层苔藓纤维发芽两实验组均有增加 ,但P60 (3 .2 5±l.0 3 )较P10 (l.5± 0 .92 )更明显 ;以上指标经比较均有显著差异(P均 <0 .0 5 )。结论 1.虽幼鼠较易发生惊厥 ,但致后海马神经元损伤、病理性
Objective To observe the changes of hippocampal structure after repeated seizures in different mature rats and to study the tolerance of rats to convulsive injury. Methods P10 and P60 experimental rats were irradiated with pentylenetetrazol for 5 days, and P10 and P60 saline control groups were set up. The latency, latency and duration of convulsion in both experimental groups were observed. The hippocampal nerve Morphological changes: hippocampal CA1, CA3, DG and portal area cell count; histochemical staining of mossy fiber germination, respectively, experimental groups, the experimental group and the corresponding control group comparison. Compared with P60 [(8.27 ± 1.48), (4.16 ± 5.2, P <0.05), the latent period of P10 convulsion seizure was (1.07 ± 0.55) 2) min], duration of convulsion (4 6.33 ± 7.65) min was significantly longer than that of adult rats (17.0 ± 5.46) min. 2.Compared with control group, Significant loss of neurons, dentate gyrus DG number of granulocytes in the experimental group (23.5 ± 3.60) than the control group (16.2 5 ± 1.58) increased. Compared with the control group (6.31 ± 1.5 0,3 .62 ± 1.40), the number of neurons in CA1 and CA3 area of P60 experimental group was significantly decreased (8.22 ± 1.88,5.62 ± 1.68), but there was no significant change in DG area: Timm stain CA3 pyramidal layer mossy fiber sprouting two experimental groups were increased, but P60 (3.25 ± 1.03) than P10 (1.5 ± 0.92) more obvious; the above indicators by comparison There were significant differences (P <0.05). Conclusion 1. Although convulsions are more likely to occur in young rats, hippocampal neurons damage and pathology