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为探讨血吸虫病肝纤维化的免疫学发病机理，对感染血吸虫的新西兰兔进行了动态观察。在感染后4、6、8、10、12、16、20、24、28wk，分别取肝组织活植，用定量RT－PCR法检测肝组织的细胞因子mRNA水平，并作形态学与免疫组化检查。结果认为肝脏内多种细胞因子mRNA水平与虫卵内芽肿变化呈平行关系，感染4wk后即有上升，以TGF-β_1上升最明显，在感染后第8wK达到峰值，随之下降，从12wk至16wk下降最明显。TGF-β_1和IFN－γmRNA水平的比值随肝纤维化进展而变大，可能具有较大的实用价值。本研究提示日本血吸虫虫卵肉芽肿与肝纤维化不仅具有不同的细胞学基础，而且其免疫调节作用也不同。
To investigate the immunological pathogenesis of hepatic fibrosis in schistosomiasis, dynamic observation of New Zealand rabbits infected with schistosomiasis was performed. At 4, 6, 8, 10, 12, 16, 20, 24 and 28wk after infection, the liver tissues were harvested and the mRNA levels of cytokines in the liver tissue were detected by quantitative RT-PCR. Check The results showed that the mRNA levels of various cytokines in the liver were in parallel with the change of intragluteal neovascularization. After 4 weeks of infection, the mRNA levels of TGF-β1 increased most obviously, reached the peak at 8th week after infection, and then decreased. From 12wk The most obvious drop to 16wk. The ratio of TGF-β 1 and IFN-γ mRNA levels may increase with the progression of liver fibrosis, which may be of great practical value. This study suggests that Schistosoma japonicum egg granuloma and liver fibrosis not only have different cytological basis, but also different immunomodulatory effects.