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采用细胞─酶联标记法测定了结核性胸膜炎病人胸水中Ｂ淋巴细胞ＣＤ２３、ＣＤ４０的表达，并进一步观察了Ｂ淋巴细胞在Ｔ淋巴细胞、ＰＰＤ等参与培养后ＣＤ２３、ＣＮ４０的表达受影响情况。结果显示：结核性胸膜炎病人胸水中Ｂ琳巴细胞与Ｔ淋巴细胞混合培养后与单独Ｂ淋巴细胞培养组相比较，ＣＤ２３的表达明显降低（Ｐ＜０．０５），二者存在密切相关（ｒ＝０．８０），而Ｂ＋ＰＰＤ、Ｂ＋ＰＰＤ＋Ｔ培养组与Ｂ淋巴细胞培养组相比较，ＣＤ２３表达无明显差异（Ｐ＞０．０５）；各组之间ＣＤ４０的表达无差异。健康人外周血Ｂ淋巴细胞培养过程中ＣＤ２３、ＣＤ４０的表达不受Ｔ细胞及ＰＰＤ的影响。提示：结核病人Ｔ细胞对Ｂ细胞ＣＤ２３的表达具有明显抑制作用。
The expression of CD23 and CD40 on B lymphocytes in pleural fluid of patients with tuberculous pleurisy were detected by enzyme linked immunosorbent assay (ELISA). The expression of CD23 and CN40 on B lymphocytes in T lymphocytes and PPD were further observed. The results showed that the expression of CD23 was significantly lower in tuberculous pleurisy patients than in B lymphocyte culture group (P <0.05) after mixed culture of B lymphocytes and T lymphocytes in pleural effusion = 0.80). There was no significant difference in the expression of CD23 between B + PPD, B + PPD + T group and B lymphocyte group (P> 0.05). There was no difference in the expression of CD40 between groups. The expression of CD23 and CD40 during the process of peripheral blood B lymphocyte culture in healthy volunteers was not affected by T cells and PPD. Tip: T cells of patients with tuberculosis CD23 expression of B cells had a significant inhibitory effect.