论文部分内容阅读
Objective To analyze the association between mutation(s) in preS region of HBV and hepatitis B disease progress in Chinese patients with genotype C chronic HBV infection.Methods Ninety-three patients with chronic genotype C HBV infection,including 24 asymptomatic carriers(ASC) ,26 patients with chronic hepatitis B(CHB) ,22 patients with liver cirrhosis(LC) and 21 HCC patients were investigated.Levels of HBV DNA,HBeAg,alanine aminotransferase(ALT) ,asparate transaminase(AST) were measured.HBV preS region was analyzed by PCR direct sequencing.Results The prevalence of preS T3098C and T53C mutations of genotype C HBV was significantly higher in LC and HCC patients than ASC and CHB patients.The rate of T3098C mutation in ASC,CHB,LC,and HCC patients were 0.00%(0/24) ,3.85%(1/26) ,9.09%(2/22) ,and 30.77%(8/22) ,respectively(P=0.0015) ,while the rate of T53C mutation was 12.50%(3/24) ,3.85%(1/26) ,40.91%(9/22) ,and 42.31%(11/26) ,respectively(P=0.0012) .Conclusion The frequency of genotype C HBV preS T3098C and T53C mutations is associated with hepatitis B infection progression.
Objective To analyze the association between mutation (s) in preS region of HBV and hepatitis B disease progression in Chinese patients with genotype C chronic HBV infection. Methods Ninety-three patients with chronic genotype C HBV infection, including 24 asymptomatic carriers (ASC), 26 patients with chronic hepatitis B (CHB), 22 patients with liver cirrhosis (LC) and 21 HCC patients were investigated. Levels of HBV DNA, HBeAg, alanine aminotransferase (ALT), asparate transaminase analyzed by PCR direct sequencing. Results of the prevalence of preS T3098C and T53C mutations of genotype C HBV was significantly higher in LC and HCC patients than ASC and CHB patients. rate of T3098C mutation in ASC, CHB, LC, and HCC patients were 0.00 % (0/24), 3.85% (1/26), 9.09% (2/22), and 30.77% (8/22) respectively (P = 0.0015), while the rate of T53C mutation was 12.50% / 24), 3.85% (1/26), 40.91% (9/22), and 42.31% (11/26), respectively (P = 0.0012) .Conclusion The frequency of genotype C HBV preS T3098C and T53C mutations are associated with hepatitis B infection progression.