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研究了不同剂量12C6+离子辐照对中兰1号、BC-04-477、塔城3种苜蓿M1代个体在低温胁迫下存活率、过氧化氢酶(CAT)及过氧化物酶(POD)活性的影响。在辐照剂量为400 Gy时,中兰1号低温胁迫组存活率、CAT活性比未辐照的对照分别提高了33.3%,56.3%,POD活性与未辐照的对照无差异;在辐照剂量为400 Gy时,BC-04-477低温胁迫组存活率、CAT及POD活性比未辐照的对照组分别提高了33.3%,69.2%,5.1%;塔城在辐照剂量为800 Gy时,低温胁迫组的存活率、CAT及POD活性比未辐照的对照组分别提高了25%,26%,22.8%。以上结果表明,12C6+离子辐照可以提高中兰1号、BC-04-477、塔城的低温环境的存活能力,提高苜蓿抗寒性能。
The survival rate, the activities of catalase (CAT) and peroxidase (POD) in M1 plants of Zhonglian 1, BC-04-477 and Tacheng alfalfa were studied under different doses of 12C6 + ion irradiation. Effect of activity. When the irradiation dose was 400 Gy, the survival rate and CAT activity of Zhonglan 1 under cold stress were increased by 33.3% and 56.3%, respectively, compared with that of non-irradiated control. There was no difference in POD activity between the two treatments At the dose of 400 Gy, the survival rate, CAT and POD activities of BC-04-477 low temperature stress group were increased by 33.3%, 69.2% and 5.1% respectively compared with those of non-irradiated control group. When the radiation dose was 800 Gy , The survival rate, CAT and POD activity of the low temperature stress group were increased by 25%, 26% and 22.8% respectively compared with the unirradiated control group. The above results show that 12C6 + ion irradiation can improve the survival ability of Zhonglan 1, BC-04-477 and Tacheng in low temperature environment and improve the cold resistance of alfalfa.