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目的指示球形幽门螺杆菌致细胞空泡变性毒力的变异情况。方法采用延期培养和加亚抑菌浓度抗生素，对细胞毒相关蛋白基因阳性（cagA＋），和细胞空泡毒素基因阳性（vacA＋）的高毒株幽门螺杆菌（Hp）进行球形诱变，进而检测该Hp至Hela细胞空泡变性的毒力，并用SDS-PAGE、PCR及PCR-SSCP技术，分析球形Hp蛋白产物及决定细胞空泡变性的cagA和vacA基因的变异情况。结果与结论球形Hp致细胞空泡毒力减弱，74KD以上的蛋白含量减少，特别是125KD条带尤为明显。259bp的vacA基因片段和349bpcagA基因片段未发生缺失，但两种方法诱变的球形HpvacA基因均存在点突变。
Objective To indicate the variation of virulence of cell vacuolar degeneration induced by Helicobacter pylori. Methods Sporadic mutagenesis and antibacterial concentrations of antibacterials were used to detect the mutated Helicobacter pylori (Hp), which was positive for cagA + and vacA + The Hp to Hela cell vacuolar degeneration virulence, and by SDS-PAGE, PCR and PCR-SSCP technology, analysis of spherical Hp protein products and cell vacuolar degeneration of cagA and vacA gene variation. RESULTS AND CONCLUSION: The virulence of vacuolar Hp-deficient cells was weakened and the content of proteins above 74 kD decreased, especially in 125 kD bands. VacA gene fragment and 349bpcagA gene fragment of 259bp were not deleted, but there were point mutations in the spherical HpvacA gene mutated by both methods.