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目的 探讨锰对正常人外周血淋巴细胞微核率和姊妹染色单体交换（SCE）频率的影响。方法 用不同浓度的硫酸锰溶液（培养液中锰浓度:0.05、0.15、0.25、0.75、1.50μg/ml）处理体外培养的人外周血淋巴细胞,观察其微核率和SCE频率的变化,同时以生理盐水为对照。结果 锰剂量≤0.25μg/ml时,实验组与对照组相比,其微核率和SCE频率变化均不显著（P>0.05）;锰剂量≥0.75μg/ml时,微核率和SCE频率均显著高于对照组（P<0.01）,且呈剂量-反应关系。结论0.25μg/ml以下低剂量锰对细胞遗传物质是比较安全的,而0.75μg/ml以上高剂量锰可造成不同类型DNA损伤。
Objective To investigate the effects of manganese on the frequencies of micronuclei and sister chromatid exchange (SCE) in normal human peripheral blood lymphocytes. Methods The human peripheral blood lymphocytes cultured in vitro were treated with different concentrations of manganese sulfate solution (manganese concentration in culture medium: 0.05,0.15,0.25,0.75,1.50μg / ml), and observed the change of micronucleus rate and SCE frequency With saline as a control. Results Compared with the control group, the micronucleus rate and SCE frequency did not change significantly (P> 0.05) when the manganese dose was less than 0.25μg / ml. The micronucleus rate and SCE frequency Were significantly higher than the control group (P <0.01), and the dose-response relationship. Conclusion Low dose of 0.25μg / ml of manganese is more safe for cytogenetic substances, and high dose of 0.75μg / ml of manganese can cause different types of DNA damage.