Generation of Transgene-free Induced Pluripotent Stem Cells with Non-viral Methods

• 作 者: Tao Wang Hua-shan Zhao Qiu-lin
• 机 构: InstituteofMolecularBiology,ThirdClinicalMedicalSchool,FirstClinicalMedicalSchool,DepartmentofNeuros
• 出 处: 中国医学科学杂志：英文版
• 发表日期: 2013年1期
• 关键词: 多能干细胞 病毒 诱导 转基因 骨髓间充质干细胞 C-MYC基因 成纤维细胞 移植治疗 induced pluripotent stem cells non-v

Induced pluripotent stem(iPS) cells were originally generated from mouse fibroblasts by enforced expression of Yamanaka factors(Oct3/4,Sox2,Klf4,and c-Myc).The technique was quickly reproduced with human fibroblasts or mesenchymal stem cells.Although havi