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本实验采用组织血流量计法，对正常和感染的ＳＤ大鼠末端回肠，进行了肠粘膜下血流量的测定，以观察厌氧灵对小肠血流量的影响。为了明确厌氧灵对内毒素体外诱生的单核／巨噬细胞分泌细胞因子ＴＮＦ、ＩＬ—１、ＩＬ—６的影响，采用细胞培养技术，分别观察了厌氧灵、氢化可的松对人外周血单核细胞及小鼠腹腔内巨噬细胞培养上清液由内毒素刺激的ＴＮＦ、ＩＬ—１、ＩＬ—６的诱生作用。实验结果表明，腹膜炎大鼠的肠血流量（５６．１５±７．６６ｍｌ／ｍｉｎ／１００ｇ）较正常大鼠（７１．１６±６．１９ｍｌ／ｍｉｎ／１００ｇ）明显降低（Ｐ＜０．０１）；对于正常大鼠，投予厌氧灵后６０、９０分钟的肠血流量（９４．２６±１３．０２ｍｌ／ｍｉｎ／１００ｇ，８５．７４±５．５８ｍｌ／ｍｉｎ／１００ｇ）与投药前生理盐水、先锋Ⅳ、灭滴灵比较均显著升高（ｐ＜０．０５）；对于腹膜炎大鼠，投予厌氧灵后，６０、９０、１２０分钟的肠血流量（７４．８５±１７．５３ｍｌ／ｍｉｎ／１００ｇ，８２．ｌｌ±１２．５３ｍｌ／ｍｉｎ／１００ｇ，６４．８９±６．７７ｍｌ／ｍｉｎ／１００ｇ）明显高于投药前（５５．２９±７．０６ｍｌ／ｍｉｎ／１００ｇ），６０、９０分钟肠血流量亦显著高于生理?
In this study, tissue flowmeter method was used to measure the intestinal submucosal blood flow in the terminal ileum of normal and infected SD rats to observe the effect of anaerobic on small intestine blood flow. In order to clarify the effect of anaerobic on the secretion of cytokines TNF, IL-1 and IL-6 of monocytes / macrophages induced by endotoxin in vitro, the cell culture techniques were used to observe the effects of anorexia and hydrocortisone Human peripheral blood mononuclear cells and mouse peritoneal macrophages culture supernatants stimulated by endotoxin TNF, IL-1, IL-6 induced effect. The experimental results showed that the intestinal blood flow (56.15 ± 7.66ml / min / 100g) in peritonitis rats was significantly lower than that in normal rats (71.16 ± 6.19ml / min / 100g) (P <0.01) ; For normal rats, intestinal blood flow (94.26 ± 13.02ml / min / 100g, 85.74 ± 5.58ml / min / 100g) at 60 and 90 minutes after anaerobic administration was similar to that before administration of normal saline , Vanguard Ⅳ, metronidazole were significantly higher (p <0.05); for the peritonitis in rats after anorexia after 60,90,120 minutes of intestinal blood flow (74.85 ± 17.53ml /min/100g, 82. 21 ± 12.53ml / min / 100g, 64.89 ± 6.77ml / min / 100g) was significantly higher than that before administration (55.29 ± 7.06ml / min / 100g) Intestinal blood flow was significantly higher in 90 minutes than in physiology?