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目的:幼年时期营养过剩,不仅会引起肥胖,还会影响青春期的启动、发育和以后的生殖机能。我们以8周的耐力游泳为运动模型,探讨耐力运动对高脂膳食雄性大鼠脂肪组织OB-RmRNA的基因表达和性发育的影响,诣在为揭示运动减肥机制以及与性发育的关系提供实验依据。方法:Wistar雄性大鼠40只,随机分三组:普通膳食组(C)、高脂膳食不运动对照组(NE)、高脂膳食运动组(E),NE和E组进行为期8周的高脂膳食和游泳训练。采用测量法测定其脂肪垫和体重,推测Lees指数,放免法检测血清瘦素、睾酮水平,分子生物学的方法测定脂肪中OB-RmRNA基因表达。结果:1)与C组相比:8周的高脂饮食使NE组体重、Lees指数、脂肪垫、血瘦素显著升高(P<0.001;P<0.01;P<0.01;P<0.01);血睾酮水平显著下降,P<0.05;NE组RNA相对含量下降59.2%;E组下降36.2%。2)与NE组相比:8周的游泳训练使E组体重、Lees指数、脂肪垫、血瘦素水平显著下降(P<0.001;P<0.01;P<0.01;P<0.05),血睾酮水平显著升高P<0.05;以NE组为对照:E组RNA相对含量上升56.2%。3)C组与E组各指标之间没有显著性差异。结论:1)高脂膳食雄性肥胖大鼠体内出现瘦素抵抗,其机制是由于瘦素受体的基因表达下调,导致其对瘦素的敏感性下降,并影响到其性发育。2)耐力训练可有效的降低体脂,上调瘦素受体的基因表达,改善瘦素抵抗,有效的缓解了肥胖引起的性发育不良。
OBJECTIVE: Excessive nutrition in childhood not only causes obesity but also affects the onset, development and subsequent reproductive function of adolescence. We take 8 weeks of endurance swimming as a sports model to explore endurance exercise on adipose tissue OB-RmRNA gene expression and sexual development in fat-fed male rats, Yi in order to reveal the mechanism of exercise to lose weight and sexual development with the experimental in accordance with. Methods: Forty Wistar male rats were randomly divided into three groups: normal diet group (C), high fat diet non-exercise control group (NE), high fat diet exercise group (E), NE and E groups for 8 weeks High fat diet and swimming training. The fat pad and body weight were measured by measurement, Lees index was presumed, serum leptin and testosterone levels were determined by radioimmunoassay, and OB-RmRNA gene expression in adipose tissue was determined by molecular biology method. Results: 1) Compared with group C, the body weight, Lees index, fat pad and leptin in NE group were significantly increased (P <0.001; P <0.01; P <0.01; P <0.01) ; Blood testosterone levels decreased significantly, P <0.05; NE relative RNA content decreased 59.2%; E group decreased 36.2%. 2) Compared with NE group, 8-week swimming training significantly decreased the body weight, Lees index, fat pad and blood leptin level (P <0.001; P <0.01; P <0.01; The level was significantly increased P <0.05; with NE as control: the relative content of E group increased 56.2%. 3) There is no significant difference between each index of C group and E group. Conclusion: 1) The leptin resistance occurs in high fat diet male obese rats. The mechanism is that the gene expression of leptin receptor is downregulated, leading to the decrease of its sensitivity to leptin and affecting its sexual development. 2) Endurance training can effectively reduce body fat, upregulate leptin receptor gene expression, improve leptin resistance and effectively alleviate obesity-induced sexual dysplasia.