论文部分内容阅读
本研究根据香味基因(badh2-E2)与非香基因(Badh2)在第2外显子7个脱氧核苷酸序列缺失差异,建立了一个新的STS分子标记和检测方法;并根据badh2-E2与Badh2+310 bp位点脱氧核苷酸的差异性,建立一个新的CAPS(EheⅠ)分子标记和检测方法。结果显示:建立的STS分子标记与前人的报道相比扩增的条带更小,只有100 bp左右,能够更加清晰地鉴别出香与非香基因型之间的差异;而建立的CAPS(EheⅠ)分子标记,纯合非香稻可获得长度为292 bp和375 bp两条带,纯合香稻可获得长度为667 bp一条带,杂合F1植株可获得长度为292 bp、375 bp和667 bp3条带,对于不同基因型检测结果的判断效果更明显。采用本研究建立的方法能够提高水稻badh2-E2类型香味基因选择效率。
In this study, a new STS molecular marker and detection method was established based on the differences of the deoxynucleotide sequences of the second exon of badh2-E2 and Badh2. According to the results of badh2-E2 With the difference of deoxynucleotide of Badh2 + 310 bp, a new CAPS (EheⅠ) molecular marker and detection method was established. The results showed that compared with the previous reports, the amplified bands of STS markers were smaller than only about 100 bp, and the differences between sweet and non-sweet genotypes could be identified more clearly. However, the established CAPS ( Ehe I). Two bands of 292 bp and 375 bp were obtained from homozygous non-fragrant rice, and a single band of 667 bp was obtained from homozygous fragrant rice. The length of hybrid F1 was 292 bp and 375 bp, 667 bp3 band, for different genotypes test results to determine the effect is more obvious. The method established in this study can improve the genotype selection efficiency of badh2-E2 type rice.