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从健康孕妇水囊引产４─６个月龄的胎儿取肝，采用ＬａＢｒｅｃｑｕｅ方法提取人肝刺激因子（ｈＨＳＳ）。经３Ｈ－胸腺嘧啶核苷参入肝ＤＮＡ法测定其生物活性。表明此ｈＨＳＳ可刺激肝细胞ＤＮＡ合成。采用皮下注射ＣＣｌ４和饮用１０％乙醇来制备慢性肝损伤动物模型，观察了ｈＨＳＳ的保护肝脏作用。结果表明：ｈＨＳＳ可使ＣＣｌ４－乙醇所致慢性肝损伤大鼠的死亡率、血清谷丙转氨酶水平、肝组织中羟脯氨酸含量的升高以及肝组织中丙二醛的含量降低。肝组织切片表明：ｈＨＳＳ能减轻肝组织的损伤程度，促进肝细胞再生，并能明显防止肝纤维化的形成和发展。可见，ｈＨＳＳ对ＣＣｌ４－乙醇所致的慢性肝损伤大鼠有明显的保护作用，其机制可能与促进肝细胞再生及抑制肝细胞膜的脂质过氧化有关。
The fetuses from 4 to 6 months of age were drawn from the healthy pregnant women’s water sac, and human liver stimulating factor (hHSS) was extracted by LaBrecque method. The biological activity of 3H-thymidine incorporation into liver DNA was measured. This hHSS can stimulate DNA synthesis in liver cells. Animal models of chronic liver injury were prepared by subcutaneous injection of CCl4 and drinking 10% ethanol, and the liver protection effect of hHSS was observed. The results showed that: hHSS could reduce the mortality, serum alanine aminotransferase, hydroxyproline content in liver tissue and the content of malondialdehyde in liver tissue of CCl4-ethanol-induced chronic liver injury rats. Liver slices showed that: hHSS can reduce the degree of liver damage, promote liver cell regeneration, and can significantly prevent the formation and development of liver fibrosis. Visible, hHSS on CCl4-ethanol-induced chronic liver injury rats have a significant protective effect, the mechanism may be to promote liver cell regeneration and inhibition of liver cell membrane lipid peroxidation.