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目的筛选弥漫大B细胞淋巴瘤(DLBCL)患者尿液中有诊断价值的挥发性标志物(VOCs)。方法收集35例DLBCL患者尿液样本(DLBCL组),30例健康者尿液样本(对照组)。利用顶空气体固相微萃取联用气相色谱-质谱检测分析两组尿液样本中的挥发性有机化合物。应用Wilcoxon非参数检验分析筛选DLBCL患者尿液中的特异性VOCs。结果筛选出正己烷、2-丁酮和4-庚酮3种特异性挥发性标志物。DLBCL组正己烷的浓度为(63.45±9.98)μg/ml,2-丁酮的浓度为(105.43±12.81)μg/ml,4-庚酮的浓度为(162.56±25.38)μg/ml。3种物质的浓度在DLBCL组和对照组之间差异有统计学意义(P<0.05)。结论 DLBCL患者尿液中挥发性化合物(正己烷、2-丁酮和4-庚酮)可能是有诊断价值的VOCs。
Objective To screen for the diagnostic value of volatile organic compounds (VOCs) in the urine of patients with diffuse large B-cell lymphoma (DLBCL). Methods Totally 35 urine samples of DLBCL patients (DLBCL group) and 30 healthy urine samples (control group) were collected. Volatile organic compounds in two groups of urine samples were analyzed by headspace gas-solid phase microextraction coupled with gas chromatography-mass spectrometry. Wilcoxon nonparametric test was used to screen urine samples for specific VOCs in DLBCL patients. Results Three specific volatile markers of n-hexane, 2-butanone and 4-heptanone were screened out. The concentration of n-hexane in DLBCL group was (63.45 ± 9.98) μg / ml, the concentration of 2-butanone was (105.43 ± 12.81) μg / ml and the concentration of 4-heptanone was (162.56 ± 25.38) μg / ml. The concentrations of three substances in the DLBCL group and the control group, the difference was statistically significant (P <0.05). Conclusions Volatile compounds (n-hexane, 2-butanone and 4-heptanone) in urine of patients with DLBCL may be valuable diagnostic VOCs.