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植物名称:油菜(Brassica campestris)。材料类别:幼嫩花蕾。培养条件:以MS为基本培养基,添加不同生长调节剂。(1)诱导丛生芽为MS+BA0.5mg/L(单位下同);(2)诱导生根为1/2MS+NAA1。上述培养基分别附加3％蔗糖,0.6％琼脂,pH6.0,温度26±2℃,每日照光约10～12小时,光照度2000lx。生长与分化情况:将未开展的幼嫩花蕾从植株上剪下,经常规消毒后接种于培养基(1)中。培养至20天时,花蕾及花柄肿胀膨大,从花萼、花瓣、花柱、
Plant name: Brassica campestris. Material category: young buds. Culture conditions: MS as the basic medium, add different growth regulators. (1) Induction of clustered shoots MS + BA0.5mg / L (the same below); (2) induced rooting is 1 / 2MS + NAA1. The above medium was supplemented with 3% sucrose, 0.6% agar, pH6.0, temperature 26 ± 2 ℃, daily light for about 10 to 12 hours, illumination 2000lx. Growth and Differentiation: Young buds that have not been cultivated are cut from the plants and routinely disinfected and then inoculated into the medium (1). When cultured to 20 days, buds and flower stem swelling swollen, from calyx, petals, styles,