mRNA Expression of Chemokine Receptors on Peripheral Blood Mononuclear Cells and Correlation with Cl

• 作 者: Yu-mei Li Zhi-qiang Chen Xu Ya
• 机 构: Department of Dermatology,Hospital for Skin Diseases,Central Lab of People's Liberation Army Cancer
• 出 处: 中国医学科学杂志（英文版）
• 发表日期: 2004年期
• 关键词: systemic lupus erythematosus chemokine receptors peripheral blood mononuclear ce

Objective To investigate the expressions of chemokine receptors and interleukin (1L) receptors on the peripheral blood mononuclear cells (PBMCs) from systemic lupus erythematosus (SLE) patients and their correlations with clinical features as well as SLE disease activity index (SLEDAI).Methods The mRNA expressions of ehemokine receptors and IL receptors on PBMCs of 93 SLE patients and 30 healthy controls were detected by reverse transcription-polymerase chain reaction, including CCR2, CCR3, CCR4, CCRS, CCR6, CCR8, CXCR3, CXCRS, CX3CR1, XCR1, IL-4R, and IL-10R. The clinical features of SLE patients were recorded. The correlations of chemokine receptors and IL receptors mRNA expressions with clinical features as well as SLEDAI were assayed using linear regression analysis.Results The level of CCR5 mRNA in SLE patients (including active and inactive SLE) was signifi-cantly higher than that in healthy controls (P0.05). CX3CR1 mRNA expression significantly increased from healthy control to inactive SLE to active SLE in sequence. The others (except for CCR8, CXCR3, and IL-10R) in active SLE patients were significantly higher than those in both inactive SLE patients and healthy controls (all P