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通过16S rDNA扩增产物限制性片段长度多态性分析(ARDRA),对兰坪铅锌尾矿区豆科植物根瘤菌的遗传多样性进行了研究。采用限制性内切酶Hae Ⅲ、Hind Ⅲ、Hinf Ⅰ和Taq Ⅰ对16S rDNA扩增产物进行了酶切分型,根据ARDRA酶切图谱的不同,进行树状聚类。结果表明:49株根瘤菌在40%的相似水平上按氮含量不同及铅锌含量的采集地不同分别聚为OTU1、OTU2和OTU33个群,说明根瘤菌的遗传多样性及分布与土壤中的氮含量和铅锌含量有关。代表菌株的16S rDNA测序结果分析表明,它们在系统发育树上属于Rhizobium sp.、Sinorhizobium sp.和Bradyrhizobium sp.3个系统发育分支,进一步说明兰坪铅锌尾矿区豆科植物根瘤菌多样性较丰富。
The genetic diversity of legume Rhizobium strains in the Lanping lead-zinc tailing area was studied by restriction fragment length polymorphism analysis (ARDRA) of 16S rDNA amplification products. 16S rDNA amplification products were digested by restriction endonucleases Hae Ⅲ, Hind Ⅲ, Hinf Ⅰ and Taq Ⅰ. According to the different ARDRA digestion patterns, dendrogram clustering was carried out. The results showed that 49 Rhizobium strains were clustered into OTU1, OTU2 and OTU33 groups at 40% similarity level according to different nitrogen content and lead-zinc content, indicating that the rhizobia genetic diversity and distribution were similar to those in soil Nitrogen content and lead and zinc content. The results of 16S rDNA sequencing of representative strains showed that they belonged to three phylogenetic branches of Rhizobium sp., Sinorhizobium sp. And Bradyrhizobium sp in phylogenetic tree, which further indicated that rhizobium diversity of leguminous plants in Lanping lead-zinc tailings area was more rich.