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目的 :用化学发光法测定三勒浆口服液中SOD活性并消除样品中抗坏血酸等成分的干扰。方法 :分别用黄嘌呤 黄嘌呤氧化酶及邻苯三酚自氧化作为酶和非酶超氧阴离子自由基发生体系 ,以鲁米诺为发光剂 ,先测定样品对发光的总抑制率 ,并通过加热样品使SOD失活测得样品发光抑制率本底值 ,从样品对发光的总抑制率 ,扣除本底即得SOD活性。结果 :在两种体系中测得结果有高度一致性 ,样品SOD活性分别为 (170± 9 3)U·ml 1和 (16 5± 2 0 )U·ml 1。结论 :该法可用于测定生物制剂中SOD的活性 ,方便有效地消除本底的干扰 ,不需有机溶剂萃取 ,是一种实用、可靠的测定SOD活力的方法
OBJECTIVE: To determine the SOD activity in SanLiLi oral liquid by chemiluminescence and eliminate the interference of ascorbic acid and other components in the sample. Methods: Xanthine xanthine oxidase and pyrogallol autoxidation were used as free radical generating system for enzyme and non-enzyme superoxide anion respectively. Luminol was used as luminant to determine the total inhibition rate of luminescence. The sample was heated to inactivate SOD to determine the background value of the luminescence inhibition rate. The total inhibition of luminescence from the sample was subtracted from the background to obtain the SOD activity. Results: The results were highly consistent across the two systems. The SOD activity was (170 ± 9 3) U · ml 1 and (16 5 ± 20) U · ml 1, respectively. Conclusion: The method can be used to determine the activity of SOD in biological agents, to eliminate background interference easily and effectively without organic solvent extraction. It is a practical and reliable method to determine SOD activity