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植物名称:草木樨状黄芪(Astragalus melilo-toides)。材料类别:下胚轴。培养条件:(1)三角瓶内铺一薄层脱脂棉,上盖滤纸,以蒸馏水浸润高压灭菌后备用;(2)MS+2,4-D1.0～2.0mg/L(单位下同);(3)MS+BA0.5～2.5+LH500;(4)MS+IBA0.5～1.0。所有MS培养基均附加蔗糖3％并以0.6％的琼脂固化,pH5.6～5.8,培养温度25±2℃,每天光照12小时,光照度1200lx。生长与分化情况:种子经常规灭菌后于(1)中萌
Plant name: Astragalus Astragalus (Astragalus melilo-toides). Material Type: Hypocotyl. (2) MS + 2,4-D1.0 ~ 2.0mg / L (same unit the same below). The culture conditions: (1) triangular flask with a thin layer of absorbent cotton, filter paper on the cover, immersed in distilled water for sterilization; ; (3) MS + BA0.5 ~ 2.5 + LH500; (4) MS + IBA0.5 ~ 1.0. All MS medium was supplemented with sucrose 3% and solidified with 0.6% agar, pH5.6 ~ 5.8, culture temperature 25 ± 2 ℃, light 12 hours a day, light intensity 1200lx. Growth and differentiation: seeds after routine sterilization (1) Meng