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[目的]研究蝴蝶干标本DNA的提取方法。[方法]采用5种不同的方法(SDS法、SDS-巯基乙醇-酚法、CTAB法、饱和NaCl法、SDS-巯基乙醇-氯仿法)对蝴蝶干标本DNA进行提取。[结果]SDS-巯基乙醇-酚法、饱和NaCl法和SDS-巯基乙醇-氯仿法适合蝴蝶干标本基因组DNA的提取,并且能成功地应用于蝴蝶线粒体COI、EF-1α和CytB基因的PCR扩增。[结论]SDS-巯基乙醇-酚法、饱和NaCl法和SDS-巯基乙醇-氯仿法可以用于研究蝴蝶干标本DNA的抽提,为蝴蝶的分子系统进化研究奠定了基础。
[Objective] The research aimed to study the DNA extraction method of butterfly dry specimens. [Method] Five kinds of different methods (SDS method, SDS-mercaptoethanol-phenol method, CTAB method, saturated NaCl method and SDS-mercaptoethanol-chloroform method) [Result] SDS-mercaptoethanol-phenol method, saturated NaCl method and SDS-mercaptoethanol-chloroform method were suitable for the extraction of genomic DNA from butterflies and were successfully applied to the PCR amplification of butterfly mitochondrial COI, EF-1α and CytB genes increase. [Conclusion] SDS-mercaptoethanol-phenol method, saturated NaCl method and SDS-mercaptoethanol-chloroform method could be used to study the extraction of DNA from dried butterfly specimens, which laid the foundation for the molecular evolution of butterfly.