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目的探讨亚甲基四氢叶酸还原酶(MTHFR)基因C677T变异及其与环境危险因素交互作用对食管癌发病的影响。方法采用病例-对照研究方法,以新诊断的食管癌患者为研究对象,用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)检测MTHFR基因C677T变异,运用多因素Logistic回归分析MTHFR基因C677T变异与环境危险因素交互作用对食管癌发病的影响。结果MTHFR基因C677T变异与饮酒指数、热烫饮食、霉变食品、精神状态和年龄间存在相乘模型的协同作用,OR值依次为10.892(P=0.013 8),12.043(P=0.012 4),16.444(P=0.009 6),20.581(P≤0.000 1),5.201(P=0.014 5)。结论MTHFR基因C677T变异可增加老年人、大量饮酒、热烫饮食、霉变食品和精神状态不良等危险因素者患食管癌的危险性。
Objective To investigate the effect of the interaction between methylenetetrahydrofolate reductase (MTHFR) gene C677T and its interaction with environmental risk factors on the pathogenesis of esophageal cancer. Methods A case-control study was conducted in newly diagnosed esophageal cancer patients. The C677T mutation of MTHFR gene was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Multivariate Logistic regression Analysis of the interaction between MTHFR C677T mutation and environmental risk factors on the incidence of esophageal cancer. Results The synergistic effect of MTHFR gene C677T mutation with alcohol consumption index, blanching diet, mildew food, mental state and age was found. The OR values of MTHFR gene C677T were 10.892 (P = 0.013 8), 12.043 (P = 0.012 4) 16.444 (P = 0.009 6), 20.581 (P ≦ 0.0001), 5.201 (P = 0.014 5). Conclusion The C677T mutation of MTHFR gene may increase the risk of esophageal cancer in the elderly, heavy drinking, blanching diet, moldy foods and poor mental status.