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作者已研究茶树的摘除第二次新芽后,老叶中的氮素活化作用,测定了叶绿素、游离氨基酸、咖啡碱、总蛋白、可溶性蛋白、核糖体的含量,以及蛋白酶的活性。叶中氮素的损失,主要应归咎于总蛋白组分的减少。在发芽期间,可溶性蛋白含量明显下降,以后又逐渐恢复。总蛋白和可溶性蛋白的损失量分别为36％和54％。摘芽处理能部分地减少这些组分含量的下降程度。在第1、2片叶开放时,老叶中的核糖体含量随着发芽而开始作微量下降,以后又迅速恢复。摘除新芽后,蛋白酶的活性增强。但蛋白酶活性的变化与可溶性蛋白含量的损失之间没有关系或关系很小。
The authors have studied the activation of nitrogen in the old leaves after the second sprouts were removed from the tea tree, and the contents of chlorophyll, free amino acids, caffeine, total protein, soluble protein, ribosomes and protease activities were measured. The loss of nitrogen in leaves is mainly due to the reduction of the total protein fraction. During germination, soluble protein content decreased significantly, and then gradually recovered. Total protein and soluble protein loss were 36% and 54% respectively. The pick-bud treatment can partly reduce the degree of decline in the content of these components. When the first and second leaves were opened, the content of ribosomes in the old leaves started to decrease slightly as they germinated, and then rapidly recovered. After removal of shoots, protease activity increased. However, changes in protease activity have little or no relation to the loss of soluble protein content.