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为了获得药用价值较高的桑叶原料,对华东地区的5个主栽桑品种不同采摘季节、不同叶位桑叶中的总黄酮和总生物碱等主要活性物质的含量进行测定,比较这些活性物质含量在品种之间以及不同季节、不同叶位间的变化规律.测定供试桑品种桑叶样品中的总黄酮质量比在1.94～3.34 mg/g之间;总生物碱质量比在4.23～6.85 mg/g之间,其中,1-脱氧野尻霉素(DNJ)的质量比在1.89～ 3.06 mg/g之间.同一品种不同叶位桑叶中活性物质的总含量存在明显梯度,即上位叶中的含量＞中位叶中的含量＞下位叶中的含量.同一品种在不同季节采摘桑叶中的活性物质含量也存在差异:春季上、中位桑叶中的DNJ和总黄酮含量略高于秋季桑叶;总生物碱含量则是秋季桑叶高于春季桑叶.综合评价不同采样方式桑叶活性物质含量的测定结果认为,春季桑叶样品中,桑品种湖桑32号的上、中位叶及金10的下位叶中活性物质含量较高;秋季桑叶样品中,桑品种新一之濑上、中位叶及金10的下位叶中活性物质含量较高.“,”To obtain mulberry leaves of high medicinal value,the contents of total flavonoids and total alkaloids in mulberry leaves from different seasons and leaf positions of 5 mulberry varieties cultivated in east China have been determined,and the variation regularity of those bioactive substances in mulberry leaves from different varieties,seasons and leaf positions have also been investigated.The mass ratio of total flavonoids in mulberry leaves was 1.94 to 3.34 mg/g.That of total alkaloids was 4.23 to 6.85 mg/g,among which the mass ratio of 1-deoxynojirimycin (DNJ) was 1.89 to 3.06 mg/g.The total contents of these bioactive substances in different leaf positions were as follows.upper leaf＞middle leaf＞lower leaf.The contents of DNJ and total flavonoids in upper and middle mulberry leaves harvested in spring were slightly higher than those harvested in autumn,while the content of total alkaloids in mulberry leaves harvested in autumn was higher than that in mulberry leaves harvested in spring.Evaluation analysis showed that in spring,the contents of bioactive substances in upper and middle leaves of mulberry variety Husang 32 and those in lower leaves of Jin 10 were higher;in autumn,the contents of bioactive substances in upper and middle leaves of mulberry variety Shin Ichinose and those in lower leaves of mulberry variety Jin 10 were higher.