论文部分内容阅读
目的:证实西印度醋栗(Phyllanthus acidus(L.)Skeels)的果实在传统医学中作为保肝药使用的药用用途。方法:连续5d分别给予各组大鼠西印度醋栗果实的70%乙醇提取物(100,200和400mg/kg口服)及对照药水飞蓟素(100mg/kg口服)并于第2天及第3天皮下注射四氯化碳(2mL/kg)。测定血清天冬氨酸氨基转移酶(aspartate transaminase,AST)、丙氨酸氨基转移酶(alanine transaminase,ALT)、碱性磷酸酶(alkaline phosphatase,ALP)、总胆红素以及总蛋白并进行肝脏组织病理学检测。检测大鼠肝组织匀浆中的氧化应激标记物如脂质过氧化反应(lipid peroxidation,LPO)、还原型谷胱甘肽(reduced glutathione,GSH)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)以及谷胱甘肽过氧化物酶(glutathione peroxidase,GPx)以衡量西印度醋栗的体外抗氧化作用。此外,本实验还记录了伊维巴诱导的给药后小鼠睡眠时间,使用2,2-二苯基-1-苦基肼(2,2-diphenyl-1-picrylhydrazil,DPPH)法测定小鼠服药后的自由基清除率。结果:与对照组相比,西印度醋栗果实的乙醇提取物及水飞蓟素均显著降低了模型大鼠血清AST、ALT和ALP水平及LPO并升高了TP、GSH、SOD、CAT和GPx的水平(P<0.01或P<0.05)。大鼠肝脏组织的组织病理学切片结果也证实了以上实验结果。在小鼠实验中,与对照组相比,西印度醋栗果实的乙醇提取物显著缩短了伊维巴诱导的小鼠睡眠时间(P<0.01),并具有较高的DPPH清除率。结论:本研究的实验结果证实了西印度醋栗果实对四氯化碳引起的大鼠急性过氧化肝损伤的保护作用,机制可能与其抗氧化作用及自由基清除功能有关。
Objective: To confirm the medicinal use of the fruit of Phyllanthus acidus (L.) Skeels in traditional medicine as a hepatoprotective drug. Methods: 70% ethanol extract (100, 200 and 400 mg / kg orally) and control silymarin (100 mg / kg orally) were given to rats of each group for 5 days continuously and injected subcutaneously on the 2nd and 3rd day Carbon tetrachloride (2 mL / kg). Serum aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total bilirubin and total protein were measured and the liver Histopathological examination. Oxidative stress markers such as lipid peroxidation (LPO), reduced glutathione (GSH), superoxide dismutase (SOD) in rat liver homogenate ), Catalase (CAT) and glutathione peroxidase (GPx) were measured in vitro. In addition, the experiment also recorded the sleep time after ibuprofen-administered mice were treated with 2,2-diphenyl-1-picrylhydrazil (DPPH) After taking the drug free radical scavenging rate. Results: Compared with the control group, ethanol extract and silymarin of the fruits of the West Indica curcas significantly reduced the serum levels of AST, ALT and ALP, LPO and the levels of TP, GSH, SOD, CAT and GPx in the model rats (P <0.01 or P <0.05). Histopathological sections of rat liver tissue also confirmed the above experimental results. In mouse experiments, ethanol extract of Curcuma roxburghii fruit significantly reduced iweba-induced mouse sleep time (P <0.01) and had higher DPPH clearance than the control group. CONCLUSION: The experimental results in this study confirmed the protective effect of West Indian currant fruit against carbon tetrachloride-induced acute liver injury in rats, which may be related to its antioxidation and free radical scavenging function.