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目的探讨X射线对人Burkkit淋巴瘤细胞株Raji细胞增殖及凋亡的影响。方法用不同剂量X射线照射细胞,并在不同时间段用倒置显微镜观察照射后细胞的形态变化及生长抑制率,流式细胞仪检测细胞凋亡率、细胞周期,并观察X射线对克隆形成的影响。结果 16Gy照射后48h对Raji细胞的抑制率(92.67±1.20)%最显著;8Gy照射后48h早期凋亡率(42.04±3.62)%最高;8Gy照射后24h细胞周期G2/M期细胞(57.86±3.31)%,明显大于正常对照组(14.54±2.32)%;8Gy照射后第7天无克隆形成,第14天克隆数为(5.33±2.40),明显小于正常对照组第7天(116.67±20.28)和第14天(263.33±20.27)。结论 X射线可抑制细胞增殖,并诱导细胞凋亡,照射后细胞周期阻滞在G2/M期,X射线8Gy照射后不能完全抑制Raji细胞的增殖。
Objective To investigate the effect of X-ray on the proliferation and apoptosis of human Burkkit lymphoma cell line Raji. Methods The cells were irradiated with different doses of X-rays and the morphological changes and growth inhibition rates of the cells were observed by inverted microscope at different time points. The apoptosis rate and cell cycle were detected by flow cytometry. The effects of X-ray on the formation of clones influences. Results The apoptosis rate of Raji cells was the highest at 48h after irradiation with 16Gy (92.67 ± 1.20%). The apoptosis rate of Raji cells was the highest (42.04 ± 3.62)% at 48h after irradiation with 8Gy, 3.31%, which was significantly higher than that of the control group (14.54 ± 2.32)%. No clonogenicity occurred on the 7th day after 8Gy irradiation, and the number of clones on the 14th day was (5.33 ± 2.40), which was significantly lower than that of the control group on the 7th day (116.67 ± 20.28% ) And day 14 (263.33 ± 20.27). Conclusion X-ray can inhibit cell proliferation and induce apoptosis. Cell cycle arrest in G2 / M phase and X-ray irradiation after 8Gy irradiation can not completely inhibit the proliferation of Raji cells.