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以2个番茄溃疡病菌株和其它6种植物病原菌为试验材料,以micA、cytC、TomA等3个特异性基因为检测靶标,采用检测引物设计与优化、特异性测试、灵敏度测试等方法,研究建立番茄溃疡病菌的定性PCR检测方法。结果表明:依据这3个基因建立的PCR检测方法可特异、精准检测番茄溃疡病菌,其中,以TomA基因为检测靶标的方法灵敏度可达到5copy/μL或103 cfu/mL,优于另外2种方法,为番茄溃疡病菌的早期诊断提供了快速、准确的技术手段。
Two tomato canker strains and six other pathogenic fungi were used as test materials, and three specific genes, micA, cytC and TomA, were used as detection targets. The design and optimization of primers, specific tests and sensitivity tests were used to study Establishment of a qualitative PCR assay for tomato canker. The results showed that the PCR detection method based on these three genes could detect tomato canker specifically and accurately, and the sensitivity of the method was 5copy / μL or 103 cfu / mL, which was better than the other two methods , For the early diagnosis of tomato canker bacteria provides a rapid and accurate technical means.