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目的以延胡索乙素为指标成分对中药延胡索的提取工艺进行优化,以利于工业化生产。方法采用响应曲面法对提取工艺进行优化,通过考察乙醇体积分数、提取时间、溶媒用量及其交互作用对中药延胡索中延胡索乙素提取率的影响。模拟得出了延胡索乙素的提取回归方程,确定了延胡索乙素的最佳提取条件。结果优化得到的最佳工艺参数:乙醇体积分数为87.16%,提取时间为105.13 min,液料比为1∶7.13。在该工艺条件下,提取物中延胡索乙素的含量质量分数为0.183%。结论该提取工艺对延胡索提取物中的延胡索乙素提取优化效果显著,方法稳定、可行。
Objective To optimize the extraction process of Corydalis yanhusuo by using tetrahydropalmatine as an index to facilitate the industrial production. Methods The response surface methodology was used to optimize the extraction process. The effects of ethanol volume fraction, extraction time, solvent dosage and their interaction on the extraction rate of tetrahydropalmatine in Corydalis yam were studied. The regression equation of extraction of tetrahydropalmatine was obtained by simulation, and the optimal extraction conditions of tetrahydropalmatine B were established. Results The optimized process parameters were as follows: the volume fraction of ethanol was 87.16%, the extraction time was 105.13 min and the ratio of liquid to material was 1: 7.13. Under this process condition, the mass fraction of tetrahydropalmatine in the extract was 0.183%. Conclusion The extraction process of tetrahydropalmatine extract of tetrahydropalmatine significant optimization, the method is stable and feasible.