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目的：初步探讨具有活性的咽鼓管表面活性物质相关蛋白，为人工合成咽鼓管表面活性物质，提供理论依据．方法：杂色豚鼠35只活杀断头，生理盐水冲洗咽鼓管，收集冲洗液．应用氯仿／四醇革取分离为磷脂和蛋白部分，蛋白部分应用高效液相色谱（HPLC）进行分离、纯化，所得各组分
OBJECTIVE: To investigate the active Eustachian tube surfactant-related proteins and provide a theoretical basis for the synthetic synthesis of Eustachian tube surface-active substances. Methods: 35 variegated guinea pigs live kill head, saline eustachian tube, collecting irrigation fluid. Application of chloroform / tetraalcohol to separate the phospholipid and protein fractions, the protein fraction should be separated and purified by high performance liquid chromatography (HPLC), the resulting components