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1 材料和仪器 红参药材及超微粉来自北京同仁堂饮片厂。Rg1和Re购自中国药品生物制品检定所。Shimadzs LC—6B高效液相色谱仪,C—R4A积分仪。 2 实验方法 2.1 实验条件:色谱柱:Inertsil ODS3 5μΦ4.6×150mm;流动相:0.05％H_3PO_4—CH_3CN(400:99);流速:2ml/min;柱箱温度:40℃;检测波长:203nm。 2.2 提取方法:分别称取红参普通粉和超微粉各五份,每份1g,分别置于10ml具塞试管中,加水8ml。将试管放进温度为50℃的水浴中,提取时间为1、5、10、20、30分钟,提取后,离心
1 Materials and Instruments Red ginseng herbs and ultrafine powder come from Beijing Tongrentang Decoction Factory. Rg1 and Re were purchased from China National Institute for the Control of Pharmaceutical and Biological Products. Shimadzs LC-6B high performance liquid chromatograph, C-R4A integrator. 2 Experimental methods 2.1 Experimental conditions: Column: Inertsil ODS3 5μΦ4.6×150mm; mobile phase: 0.05% H_3PO_4-CH_3CN (400:99); flow rate: 2ml/min; oven temperature: 40°C; detection wavelength: 203nm. 2.2 Extraction method: Weigh five red ginseng common powders and one ultrafine powder, each 1g, and place them in 10ml plugged test tubes, respectively, and add 8ml of water. The test tube was placed in a water bath at a temperature of 50° C. The extraction time was 1, 5, 10, 20, and 30 minutes. After the extraction, centrifugation was performed.