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以电化学方法对秋水仙碱与牛血清白蛋白的相互作用进行了研究。在0.3 mol/L H2SO4底液中,秋水仙碱在玻碳电极上产生一不可逆的氧化峰,峰电位为1.18 V(vs.SCE),加入表面活性剂四丁基氯化铵后,秋水仙碱的峰信号得到明显提高。在上述条件下,加入BSA后秋水仙碱的氧化峰电位正移,峰电流下降,峰电流下降值与BSA加入的浓度在1.5×10-7～2×10-6mol/L(r=0.9978)范围内有良好的线性关系,检出限达4.0×10-8mol/L。进一步探讨了秋水仙碱与BSA的结合数和结合常数,得到结合数为1,结合常数为2.40×105L/mol。
The interaction between colchicine and bovine serum albumin was studied electrochemically. In 0.3 mol / L H2SO4 solution, colchicine produced an irreversible oxidation peak at the glassy carbon electrode with a peak potential of 1.18 V (vs.SCE). After the surfactant tetrabutylammonium chloride was added, Base peak signal was significantly improved. Under the above conditions, the oxidation peak potential of colchicine shifted positively, the peak current decreased, the peak current decreased and the concentration of BSA added was 1.5 × 10-7 ~ 2 × 10-6mol / L (r = 0.9978) Within a good linear relationship, the detection limit of 4.0 × 10-8mol / L. The binding number and binding constant of colchicine to BSA were further investigated. The binding number was 1 and the binding constant was 2.40 × 105 L / mol.