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在pH11.0的氢氧化钠介质中,有溴化十六烷基三甲基铵存在下,桑色素与铍和酒石酸形成1∶1∶2的胶束混配荧光络合物,其激发波长和发射波长分别为452nm和540nm。桑色素的检出限为8.75×10-8mol·L-1。本方法用于山楂和牡荆子中黄酮类化合物的测定,结果与用芦丁作标准的分光光度法一致,相对标准偏差分别为1.07%和1.27%
Morphine and beryllium and tartaric acid form a 1: 1: 2 micelle mixed fluorescent complex in the presence of cetyltrimethylammonium bromide at a pH of 11.0 sodium hydroxide. The excitation wavelength And emission wavelengths are 452 nm and 540 nm, respectively. The detection limit of morin is 8.75 × 10-8mol·L-1. The method was applied to the determination of flavonoids in hawthorn and vitexin. The results were consistent with the standard spectrophotometry using rutin as the standard, the relative standard deviations were 1.07% and 1.27%