Isolation and Sequence Analysis of NSP2, ORF3 and ORF5 of PRRSV XH-GD Strain from Guangdong Province

• 作 者: Zongxi CAO Quanhui PAN Zhemin
• 机 构: HainanProvincialKeyLaboratoryofTropicalAnimalReproductionandBreedingandVeterinaryMedicine,MOAKeyLabo
• 出 处: 农业生物技术：英文版
• 发表日期: 2014年1期
• 关键词: ORF5基因 PRRSV 序列分析 广东省 致细胞病变效应 逆转录聚合酶链反应 氨基酸同源性 核苷酸同源性 PRRSV XH-GD strain Seque
• 基金项目: Supported by National Natural Science Foundation of China （31272564 ） ,Joint Fund of NSFC-Guangdong of China （ U0931003 ） , China Agriculture Research System （CARS-36） ,Hainan Provincial Research Foun

The diseased samples collected from Guangdong Province were inoculated to Marc-145 cells,PRRSV-specific cytopathogenic effects( CPE) such as cell aggregation,cell shrinkage and cell exfoliation were observed and a PRRSV strain XH-GD was obtained. By using