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目的使用冷沉淀凝血因子制备仪制备冷沉淀,并与使用低温融化箱制备的冷沉淀作质量比较,从而选择合适的冷沉淀制备方法。方法将60袋新鲜冰冻血浆随机分为2组:制备仪组和融化箱组,每组30袋,1组使用冷沉淀凝血因子制备仪制备冷沉淀,另1组使用低温融化箱制备,将2组制备的冷沉淀进行质量对比。结果冷沉淀凝血因子制备仪制备的冷沉淀和低温融化箱制备的冷沉淀中Ⅷ因子含量分别为(121.80±11.87)IU和(87.27±10.03)IU,t=14.306,P<0.05;纤维蛋白原含量分别为(222.87±20.91)mg和(204.4±17.01)mg,t=4.131,P<0.05;容量分别为(45.7±2.95)m L和(45.3±2.53)m L,t=1.659,P>0.05。结论使用冷沉淀凝血因子制备仪制备的冷沉淀,不仅质量更优于低温融化箱制备的,而且在制备过程中使用了电子信息化管理,极大地提高了工作效率,制备过程更科学合理,是合适的冷沉淀制备方法。
OBJECTIVE To prepare the cryoprecipitate using the cryoprecipitate clotting factor preparation instrument and compare it with the cryoprecipitate prepared by using cryogenic thawing vessel so as to select the suitable preparation method of cryoprecipitate. Methods Sixty bags of fresh frozen plasma were randomly divided into two groups: preparation group and thawing group, each group containing 30 bags. One group was prepared using cryoprecipitate clotting factor preparation apparatus, the other group was prepared by using low temperature melting box, Group prepared by cold sedimentation mass contrast. Results The contents of Ⅷ factor in cryoprecipitate prepared by cryoprecipitate preparation and cryogenic thawing were (121.80 ± 11.87) IU and (87.27 ± 10.03) IU, respectively, t = 14.306, P <0.05; fibrinogen (P <0.05), and the contents were (222.87 ± 20.91) mg and (204.4 ± 17.01) mg, t = 4.131, P < 0.05. Conclusions The cryoprecipitate prepared by cryoprecipitate clotting factor preparation instrument is not only of better quality than cryogenic thaw box, but also used electronic information management in the preparation process, which greatly improves the working efficiency. The preparation process is more scientific and rational Suitable cryoprecipitate preparation method.