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磷酸多核糖基核糖醇(PRP)是b型流感杆菌(Hib)的荚膜多糖。PRP菌苗免疫原性弱,PRP与白喉毒素结合菌苗(PRP-D)的免疫原性明显高于PRP。作者用PRP和PRP-D分别免疫健康成人,用体内外试验比较两种菌苗诱导的抗血清的保护作用。体外调理试验是用1×10~6多形核白细胞(PMNs)/40μl与10μl对数生长期菌液[2×10~5菌落形成单位(cfu)]混合,加30μl脐带血提供补体,10μl由原倍到1∶125 5倍倍比稀释的人体PRP或PRP-D抗血清及10μl基础液,
Phosphoribosylribitol (PRP) is a capsular polysaccharide of b-type influenza bacilli (Hib). PRP vaccine immunogenicity is weak, PRP and diphtheria toxin-associated vaccine (PRP-D) immunogenicity was significantly higher than the PRP. The authors immunized healthy adults with PRP and PRP-D, respectively, and compared the antiserum protection induced by the two bacterins in vitro and in vivo. In vitro conditioning assays were performed by mixing 1 × 10 6 polymorphonuclear leukocytes (PMNs) / 40 μl with 10 μl of logarithmic growth phase [2 × 10 5 colony forming units (cfu)] plus 30 μl cord blood to provide complement and 10 μl From the original times to 1:125 5 times the ratio of human PRP or PRP-D diluted antisera and 10μl base solution,