论文部分内容阅读
目的：研究微卫星ＤＮＡｓ的遗传不稳定性与慢性粒细胞白血病（ＣＭＬ）加速、急变的关系。方法：采用基础ＰＣＲ银染方法对９例加速期和８例急变期ＣＭＬ患者的骨髓细胞与其慢性期标本位于染色体１７ｐ的Ｍｆｄ４１和１８ｑ的ＤＣＣ两个微卫星序列进行比较分析。结果：１７例ＣＭＬ加速期或急变期患者中有８例出现微卫星不稳定性（ＭＳＩ）或杂合性丢失（ＬＯＨ），占总病例数的４７．５％。６例（加速期２例，急变期４例）出现ＤＣＣ改变，占总病例数的３５．３％；２例（加速期和急变期各１例）出现Ｍｆｄ４１改变，占总病例数的１１．８％。结论：微卫星ＤＮＡｓ的遗传不稳定性可能参与ＣＭＬ加速或急变的演变
Objective: To study the relationship between the genetic instability of microsatellite DNAs and the accelerated and rapid changes of chronic myelogenous leukemia (CML). METHODS: The basic PCR-silver staining method was used to compare the two microsatellite sequences of bone marrow cells from 9 patients with accelerated stage CML and 8 cases with acute stage CML and their chronic phase samples located at chromosome 17p of Mfd41 and 18q. RESULTS: Of the 17 patients with accelerated or blastic CML, 8 cases had microsatellite instability (MSI) or loss of heterozygosity (LOH), accounting for 47.5% of the total number of cases. 6 cases (2 cases in accelerated phase and 4 cases in blast phase) had DCC changes, accounting for 35.3% of the total number of cases; 2 cases (1 case in each of acceleration phase and blast crisis phase) had Mfd41 changes, accounting for 11.1% of the total number of cases. 8%. Conclusion: The genetic instability of microsatellite DNAs may be involved in the accelerated or rapid evolution of CML