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甜菜育种或栽培实践中,常常需要进行甜菜倍性鉴定。其检查方法多利用根尖、茎尖细胞的有丝分裂或花粉母细胞减数分裂中期的染色体数目加以确定。这种方法比较细致、准确,但操作比较麻烦,且不易找到合适的分裂相。根据大量研究表明:甜菜的倍数性与花粉大小、萌发孔数目多少等有密切的相关关系。如多倍体往往比二倍体甜菜的花粉粒大、萌发孔数目较多。因此,在生产实践中,科学工作者利用简单地镜检方法测定花粉粒大小和萌发孔数目来判断甜菜植株的倍性。但是,由于甜菜花粉粒的壁较厚,并且有丰富的内含物,萌发孔数目也较多,这样给准确计算萌发孔数目带来困难。我们在观察过程中,应用了Ertman乙酰解方法处理花药后,检查花粉萌发孔得到较满意的效果。
Beet breeding or cultivation practices often require beet ploidy identification. The inspection methods and more use of apical, shoot tip cell mitosis or pollen mother cell metaphase chromosome number to be identified. This method is more detailed and accurate, but the operation is cumbersome, and not easy to find a suitable split phase. According to a large number of studies have shown that: beetle multiplicity and pollen size, the number of germination holes and so there is a close correlation. Such as polyploid tend to be larger than the diploid bee pollen grains, the number of germination holes more. Therefore, in production practice, scientists use the simple microscopic examination of pollen grain size and the number of germination holes to determine the ploidy of sugar beet plants. However, since the beet pollen grains have thick walls and abundant inclusions, the number of germination holes is also large, which makes it difficult to accurately calculate the number of germination holes. We observed the process, the application of Ertman acetylation method after processing anthers, check pollen germination hole to get more satisfactory results.