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目的探讨二甲基甲酰胺(N,N-dimethylformamide,DMF)亚慢性染毒对ICR小鼠神经行为功能的影响。方法80只ICR小鼠随机分为4组,分别以1.26、0.63、0.32 g/kg经口灌胃染毒50 d,对照组给予生理盐水(1次/d,50 d)。染毒前和染毒后分别进行Morris水迷宫、避暗箱、穿梭箱和自主活动箱等4项神经行为功能实验。结果高剂量染毒组Morris水迷宫潜伏期较染毒前时间延长,差异有统计学意义(P<0.05);避暗箱实验显示,高剂量组染毒后电击次数增加,潜伏期缩短,与染毒前比较,差异有统计学意义(P<0.05);穿梭箱实验表明,染毒组较对照组电击次数增加,其中,高剂量组与对照组比较,差异有统计学意义(P<0.05);高剂量组主动逃避时间减少,与对照组比较,差异有统计学意义(P<0.01);自主活动实验表明,染毒组较对照组自主活动增多,其中,高剂量组与对照组比较,差异有统计学意义(P<0.05)。结论亚慢性暴露于DMF对小鼠的学习记忆能力有一定的损害作用。
Objective To investigate the effects of subchronic exposure to dimethyl formamide (N, N-dimethylformamide) on neurobehavioral function in ICR mice. Methods 80 ICR mice were randomly divided into 4 groups. The mice were orally administrated with 1.26, 0.63 and 0.32 g / kg orally for 50 d, respectively. The control group was given saline (once / d, 50 d). Four neurobehavioral functional tests, Morris water maze, darkroom, shuttle box and voluntary activity box, were performed before and after the exposure. Results The latency of Morris water maze in high-dose group was longer than that in pre-exposure group (P <0.05). The dark-box experiment showed that the number of shocks in high-dose group increased and the incubation period shortened. (P <0.05). The shuttle box experiments showed that the number of shocks in the treated group increased compared with that in the control group, in which the difference between the high-dose group and the control group was statistically significant (P <0.05), and the difference was statistically significant Compared with the control group, the difference was statistically significant (P <0.01). The autonomic activity experiment showed that the activity of autophagy increased in the dose group compared with the control group, in which the difference between the high dose group and the control group was significant Statistical significance (P <0.05). Conclusion Subchronic exposure to DMF may impair the ability of learning and memory in mice.