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目的对2012年深圳市一起输入性疑似基孔肯雅热病例病原学特征进行分析。方法对疑似病例血清标本采用ELISA和荧光PCR方法分别检测基孔肯尼雅病毒Ig M、Ig G抗体和核酸,并用C6/36细胞分离病毒。采用荧光定量逆转录PCR方法扩增病毒结构蛋白基因后进行序列测定,并与不同国家和地区的毒株进行同源性比较和进化树分析。结果深圳市2012年7月报告的一起基孔肯雅热疫情为输入性病例。从病例血清标本中检测到病毒核酸,并成功分离到基孔肯尼雅病毒,将其命名为CHIKV-SZ1239。SZ1239株与非洲原型株S27-African、我国广东省2010年暴发疫情株GD05/2010、印度尼西亚2011年流行株CHIK/SBY83/11在E1基因上核苷酸同源性分别为94.0%、93.1%、98.8%。进化树显示SZ1239株与2011年新喀里多尼亚分离株NC/2011/568亲缘关系最近,其次为印度尼西亚流行株,属于Asian基因型。结论从病原学和分子生物学特征上均证实该例基孔肯雅热输入病例是由基孔肯尼雅病毒Asian基因型引起,病毒遗传特征与印度尼西亚地区流行CHIKV一致。
Objective To analyze the etiological characteristics of suspected chikungunya cases in Shenzhen in 2012. Methods Serum specimens of suspected cases were detected by ELISA and fluorescent PCR method for IgM, Ig G antibodies and nucleic acids, and C6 / 36 cells were used to isolate the virus. Fluorescent quantitative reverse transcriptase PCR was used to amplify the viral structural protein gene and then sequenced. The homology comparison and phylogenetic tree analysis were also carried out with the strains from different countries and regions. Results The chikungunya fever epidemic reported by Shenzhen City in July 2012 was an imported case. The virus nucleic acid was detected from the serum samples of the cases and the Chikungunya virus was successfully isolated and named CHIKV-SZ1239. The nucleotide homology of the E1 gene between SZ1239 strain and S27-African African prototype in China in GD05 / 2010 outbreak in 2010 and CHIK / SBY83 / 11 strain in Indonesia in 2011 were 94.0% and 93.1% respectively, 98.8%. Phylogenetic tree showed that SZ1239 strain had the closest genetic relationship with New Caledonia isolate NC / 2011/568 in 2011, followed by Indonesia, which belonged to Asian genotype. Conclusions Both etiological and molecular biological characteristics of this case of chikungunya are confirmed by the Asian genotype of Chikungunya virus and the genetic characteristics of the virus are consistent with the prevalence of CHIKV in Indonesia.